esculenta at OD 600 = 2.5 into the basal culm internode of male Jiaobai. Furthermore, an inoculation method was established to produce swollen stems by injecting a spore suspension of U. esculenta grew quickly on PDA medium at 28 ☌ in darkness. esculenta, which indicated that the ITS primers were not species-specific but that Lam16A-specific primers can distinguish U. Therefore, the septate hyphae strains were identified as U. De novo transcriptome assembly results showed that the yeast-like fungal strains did not match Ustilago, and genomic analysis suggested the septate hyphae strains were highly similar to known smut fungi. The universal primers ITS1 and ITS4 amplified a ribosomal DNA spacer element from all tested strains, while specific primers for Lam16A gene generated a 442 bp product only with septate hyphae strains. Total 220 creations.Amber Fox Pack includes 54 animals, 14 tribes, 12 city civ, 22 space civ, 7 building sets, 3 viehicle sets. esculenta, and yeast-like fungal strains and septate hyphal strains were obtained. Osiris pack includes 100 animals, 20 tribes, 20 city civilizations and 80 space creatures. The spore suspension culturing method was the best method of isolating U. ![]() The aims of this study were to investigate isolation, identification and inoculation methods of U. The smut Ustilago esculenta is an endophytic fungus that invades Zizania latifolia (Jiaobai) and plays an important role in inducing the host stem to form a swollen gall.
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